ATP1A1/2 Monoclonal antibody
ATP1A1/2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3D8G1
Cat no : 68505-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
68505-1-Ig targets ATP1A1/2 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ATP1A1/2 fusion protein Ag10489 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Calculated molecular weight | 1020 aa, 112 kDa |
| Observed molecular weight | 97-100 kDa |
| GenBank accession number | BC052271 |
| Gene symbol | ATP1A2 |
| Gene ID (NCBI) | 477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP1A1/2 antibody 68505-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
