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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat no : 66884-1-Ig

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 66884-1-Ig

    WB analysis of Jurkat using 66884-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 66884-1-Ig

    WB analysis of HSC-T6 using 66884-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66884-1-Ig

    IF Staining of HepG2 using 66884-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66884-1-Ig (ASF1A antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66884-1-Ig targets ASF1A in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC010878
Gene symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2882216
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
WB protocol for ASF1A antibody 66884-1-IgDownload protocol
IF protocol for ASF1A antibody 66884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols