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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Apolipoprotein H Monoclonal antibody

Apolipoprotein H Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

4D9A4

Cat no : 66074-1-Ig

Synonyms

Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, Beta(2)GPI, BG

Validation Data Gallery

Applications:
  • WB analysis using 66074-1-Ig

    WB analysis using 66074-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 66074-1-Ig

    WB analysis of human testis using 66074-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 66074-1-Ig

    WB analysis of human plasma using 66074-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig plasma using 66074-1-Ig

    WB analysis of pig plasma using 66074-1-Ig

    pig plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66074-1-Ig

    IHC staining of human liver cancer using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOH antibody included
  • IHC staining of human liver cancer using 66074-1-Ig

    IHC staining of human liver cancer using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOH antibody included
  • IHC staining of human colon cancer using 66074-1-Ig

    IHC staining of human colon cancer using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOH antibody included
  • IHC staining of human colon cancer using 66074-1-Ig

    IHC staining of human colon cancer using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOH antibody included
  • IHC staining of human liver using 66074-1-Ig

    IHC staining of human liver using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with APOH antibody included
  • IHC staining of human liver using 66074-1-Ig

    IHC staining of human liver using 66074-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with APOH antibody included
  • IF Staining of human liver cancer using 66074-1-Ig

    IF Staining of human liver cancer using 66074-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma, human testis tissue, pig plasma, human heart tissue, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

66074-1-Ig targets Apolipoprotein H in WB, IF, IHC, ELISA, Neutralization applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein H fusion protein Ag17915 相同性解析による交差性が予測される生物種
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC026283
Gene symbol APOH
Gene ID (NCBI) 350
RRIDAB_11182392
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein H antibody 66074-1-IgDownload protocol
IHC protocol for Apolipoprotein H antibody 66074-1-IgDownload protocol
IF protocol for Apolipoprotein H antibody 66074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Thromb Haemost

Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population.

Authors - Liang Tang
View Article
humanWB

Curr Med Sci

Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene.

Authors - Xiao-Ping Zhang
View Article
mouseWB

Biomedicines

Identification of Potential Drug Targets for Antiplatelet Therapy Specifically Targeting Platelets of Old Individuals through Proteomic Analysis

Authors - Seung Hee Lee
View Article
mouseWB,Neutralization

Crit Care

The protective effect of apolipoprotein H in paediatric sepsis

Authors - Zhicai Yu
View Article