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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOH Monoclonal antibody (66074-1-Ig)

    WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using APOH Monoclonal antibody (66074-1-Ig)

    WB analysis of human testis using 66074-1-Ig (same clone as 66074-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using APOH Monoclonal antibody (66074-1-Ig)

    WB analysis of human plasma using 66074-1-Ig (same clone as 66074-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig plasma using APOH Monoclonal antibody (66074-1-Ig)

    WB analysis of pig plasma using 66074-1-Ig (same clone as 66074-1-PBS)

    pig plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human colon cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human colon cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human liver using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOH Monoclonal antibody (66074-1-Ig)

    IHC staining of human liver using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using APOH Monoclonal antibody (66074-1-Ig)

    IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOH Monoclonal antibody (66074-1-Ig)

    IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

APOH Monoclonal antibody, PBS Only

APOH Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66074-1-PBS
Clone No.4D9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Apolipoprotein H, 4D9A4, Activated protein C-binding protein, Apo H, Apo-H

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66074-1-PBS targets APOH in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17915

Product name: Recombinant human APOH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-345 aa of BC026283

Sequence: RTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPC

相同性解析による交差性が予測される生物種
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC026283
Gene Symbol APOH
Gene ID (NCBI) 350
RRIDAB_11182392
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP02749
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

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