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- KD/KO Validated
ANP32B Monoclonal antibody
ANP32B Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5A1F12
Cat no : 66160-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, fetal human brain tissue, HEK-293 cells, mouse brain tissue, U2OS cells, A431 cells, HCT 116 cells, HeLa cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
66160-1-Ig targets ANP32B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ANP32B fusion protein Ag1274 相同性解析による交差性が予測される生物種 |
| Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member B |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC013003 |
| Gene symbol | ANP32B |
| Gene ID (NCBI) | 10541 |
| RRID | AB_2881556 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANP32B antibody 66160-1-Ig | Download protocol |
| IF protocol for ANP32B antibody 66160-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol ANP32 proteins are essential for influenza virus replication in human cells.
|
