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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for WB, IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8C5

Cat no : CL594-67687

Synonyms

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32

Validation Data Gallery

Applications:
  • WB analysis using CL594-67687

    WB analysis using CL594-67687

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67687 (ANP32A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using CL594-67687

    IF Staining of A431 using CL594-67687

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 ANP32A antibody (CL594-67687, Clone: 2D8C5 ) at dilution of 1:200, CL488-Phalloidin (green).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Raji cells
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67687-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67687 targets ANP32A in WB, IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007200
Gene symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2920172
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Protocols

Product Specific Protocols
WB protocol for CL594 ANP32A antibody CL594-67687Download protocol
IF protocol for CL594 ANP32A antibody CL594-67687Download protocol
Standard Protocols
Click here to view our Standard Protocols