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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ALDH1A1 Polyclonal antibody

ALDH1A1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15910

Synonyms

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-15910

    IF Staining of A549 using CL488-15910

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 ALDH1A1 antibody (CL488-15910) at dilution of 1:600.

Tested Applications

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 15910-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

CL488-15910 targets ALDH1A1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A1 fusion protein Ag8665 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight 501 aa, 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC001505
Gene symbol ALDH1A1
Gene ID (NCBI) 216
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ALDH1A1 antibody CL488-15910Download protocol
Standard Protocols
Click here to view our Standard Protocols