• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AIRE Monoclonal antibody

AIRE Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3C3

Cat no : CL488-66262

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66262

    FC experiment of HeLa using CL488-66262

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AIRE (CL488-66262, Clone:1A3C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66262-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66262 targets AIRE in FC (Intra) applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIRE fusion protein Ag19103 相同性解析による交差性が予測される生物種
Full Name autoimmune regulator
Calculated molecular weight 545 aa, 58 kDa
Observed molecular weight 58-69 kDa
GenBank accession numberBC137270
Gene symbol AIRE
Gene ID (NCBI) 326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 AIRE antibody CL488-66262Download protocol
Standard Protocols
Click here to view our Standard Protocols