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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E12A8

Cat no : CL488-66902

Synonyms

absent in melanoma 2, AIM2, PYHIN4

Validation Data Gallery

Applications:
  • WB analysis using CL488-66902

    WB analysis using CL488-66902

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66902 (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66902-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66902 targets AIM2 in WB applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607 相同性解析による交差性が予測される生物種
Full Name absent in melanoma 2
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 37-39 and 53 kDa
GenBank accession numberBC010940
Gene symbol AIM2
Gene ID (NCBI) 9447
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 AIM2 antibody CL488-66902Download protocol
Standard Protocols
Click here to view our Standard Protocols