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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

AFP Recombinant antibody

AFP Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D17

Cat no : 81083-1-RR

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

Validation Data Gallery

Applications:
  • WB analysis using 81083-1-RR

    WB analysis using 81083-1-RR

    human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81083-1-RR

    WB analysis of HepG2 using 81083-1-RR

    WB result of AFP antibody (81083-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells.

  • WB analysis using 81083-1-RR

    WB analysis using 81083-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 81083-1-RR

    WB analysis of HuH-7 using 81083-1-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 81083-1-RR

    IHC staining of human colon cancer using 81083-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human colon cancer using 81083-1-RR

    IHC staining of human colon cancer using 81083-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AFP antibody included

Tested Applications

Positive WB detected inHepG2 cells, human placenta tissue, HuH-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

81083-1-RR targets AFP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68-72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).

Protocols

Product Specific Protocols
WB protocol for AFP antibody 81083-1-RRDownload protocol
IHC protocol for AFP antibody 81083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols