CoraLite® Plus 488-conjugated AFM Monoclonal antibody
AFM Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G9F6
Cat no : CL488-66113
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66113-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
The immunogen of CL488-66113 is AFM Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AFM fusion protein Ag16866 相同性解析による交差性が予測される生物種 |
| Full Name | afamin |
| Calculated molecular weight | 599 aa, 69 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC109020 |
| Gene symbol | AFM |
| Gene ID (NCBI) | 173 |
| RRID | AB_2883260 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AFM antibody CL488-66113 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
