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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F1B4

Cat no : CL594-66939

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66939

    IF Staining of HepG2 using CL594-66939

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 ADH1B antibody (CL594-66939, Clone: 1F1B4 ) at dilution of 1:200, CL488-Phalloidin (green).

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66939-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66939 targets ADH1B in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADH1B fusion protein Ag10630 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC033009
Gene symbol ADH1B
Gene ID (NCBI) 125
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Protocols

Product Specific Protocols
IF protocol for CL594 ADH1B antibody CL594-66939Download protocol
Standard Protocols
Click here to view our Standard Protocols