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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADC Monoclonal antibody

ADC Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B11

Cat no : 67884-1-Ig

Synonyms

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

Validation Data Gallery

Applications:
  • WB analysis of HEK-293T using 67884-1-Ig

    WB analysis of HEK-293T using 67884-1-Ig

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67884-1-Ig

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67884-1-Ig

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 67884-1-Ig

    WB analysis of mouse brain using 67884-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67884-1-Ig

    WB analysis of HeLa using 67884-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 67884-1-Ig

    WB analysis of mouse cerebellum using 67884-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 67884-1-Ig

    WB analysis of mouse brain using 67884-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67884-1-Ig

    IF Staining of A431 using 67884-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue, HEK-293 cells, pig brain tissue
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67884-1-Ig targets ADC in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADC fusion protein Ag31049 相同性解析による交差性が予測される生物種
Full Name arginine decarboxylase
Calculated molecular weight 460 aa, 50 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC028128
Gene symbol ADC
Gene ID (NCBI) 113451
RRIDAB_2918641
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.

Protocols

Product Specific Protocols
WB protocol for ADC antibody 67884-1-IgDownload protocol
IF protocol for ADC antibody 67884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols