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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14139

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-14139

    FC experiment of HeLa using CL488-14139

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ADAM12 (CL488-14139) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14139-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-14139 targets ADAM12 in FC (Intra) applications and shows reactivity with human, mouse, Rat samples.

Tested Reactivity human, mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM12 fusion protein Ag5206 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 12
Calculated molecular weight 100 kDa
Observed molecular weight 90-100 kDa, 70-80 kDa
GenBank accession numberBC060804
Gene symbol ADAM12
Gene ID (NCBI) 8038
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ADAM12 antibody CL488-14139Download protocol
Standard Protocols
Click here to view our Standard Protocols