|Positive WB detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Sample-dependent, check data in validation data gallery|
HRP-60008 targets Beta Actin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
|Tested Reactivity||human, mouse, rat, zebrafish, plant|
|Cited Reactivity||canine, hamster, human, mouse, rat|
|Host / Isotype||Mouse / IgM|
|Full Name||actin, beta|
|Calculated molecular weight||375 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC002409|
|Gene ID (NCBI)||60|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iru (Verified Customer) (06-07-2021)
The antibody works well.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000
Hasibur (Verified Customer) (08-01-2019)
Nice antibody and no need for secondary antibody
Joanne (Verified Customer) (01-22-2019)
Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!
Tess (Verified Customer) (11-29-2018)
Little to no background, and takes half the time.