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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ACADM Monoclonal antibody

ACADM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11G6

Cat no : CL488-67742

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

Validation Data Gallery

Applications:
  • IF Staining of human liver cancer using CL488-67742

    IF Staining of human liver cancer using CL488-67742

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ACADM antibody (CL488-67742, Clone: 1A11G6 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 67742-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67742 targets ACADM in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACADM fusion protein Ag30620 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight42-47 kDa
GenBank accession numberBC005377
Gene symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2919535
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACADM antibody CL488-67742Download protocol
Standard Protocols
Click here to view our Standard Protocols