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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

1-Methyladenosine Monoclonal antibody

1-Methyladenosine Monoclonal Antibody for ELISA, Dot Blot

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

2F2H8

Cat no : 68636-1-Ig

Synonyms

1-Methyladenosine, m1A, N1-methyladenosine

Validation Data Gallery

Applications:
  • Dot Blot experiment of RNA using 68636-1-Ig

    Dot Blot experiment of RNA using 68636-1-Ig

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose.

Tested Applications

Positive Dot Blot detected inRNA

Recommended dilution

ApplicationDilution
DOT BLOTDOT BLOT : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68636-1-Ig targets 1-Methyladenosine in ELISA, Dot Blot applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTG 相同性解析による交差性が予測される生物種
Full Name 1-Methyladenosine
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).