ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1267]
Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein
Specificity
Virus
Applications
BLI, Multiplex ACE2 competition assay
Conjugate
Unconjugated
Cat no : sc-NM1267
Synonyms
Validation Data Gallery
Product Information
Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein
Specificity | Virus |
Clonality | Monoclonal |
Host | Alpaca, recombinantly produced |
Class | Recombinant |
Clone No. | NM1267 (NM1230-NM1226 fusion) [Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225] |
Type | Nanobody / VHH, biparatopic |
RRID | AB_2892257 |
Epitope | NM1230: Amino acids 351 and 437-503 (determined by X-Ray crystallography) NM1226: Amino acids 369-384 and 405, 408 and 504-508 (determined by HDX-MS) |
Immunogen | RBD of SARS-CoV-2 Spike protein |
Full Name | SARS-CoV-2 Spike Protein |
Calculated MW | 25 kDa |
GenBank Accession Number | NC_045512 |
Gene Symbol | COVID-19 S Protein |
GeneID (NCBI) | 43740568 |
Conjugate | Unconjugated |
Affinity (KD) | NM1230: 9.5 nM NM1226: 7.0 nM |
Form | Liquid |
Purification Method | Recombinant expression, affinity purification by IMAC. Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal His-tag. |
Concentration | 1000 µg/mL (34.9 µM) |
Positive Controls | BLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH |
Cited Applications | BLI, Multiplex ACE2 competition assay Note: Not recommended for Western blotting |
Storage Buffer | PBS, 0.09% sodium azide Safety datasheet (SDS): sodium azide |
Storage Condition | Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
Stability | Stable for 1 year at -20°C/-4°F after shipment. |
Shipment | Shipped at ambient temperature. |