Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-P21 (IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P21 (10355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
