HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.
1X10^6 Transfected HEK-293 cells were stained with 0.2 ug Anti-GFP tag (66002-1-Ig, Clone:1E10H7) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.
IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:2000) with the vector plasmid pEGFP-N1 Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig (GFP tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.
