PC-3 cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB result of VPS34 (C terminal) antibody (12452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 (C terminal) transfected Jurkat cells.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of PC-3 cells using 12452-1-AP (VPS34 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
