Various lysates were subjected to SDS-PAGE followed by western blot with U2AF2 mouse monoclonal antibody (68166-1-Ig) at 1:20000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig) at dilution of 1:50000. Three batches of Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EFTUD2 mouse monoclonal antibody (67855-1-Ig, isotype IgG1) at dilution of 1:10000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Flag tag mouse monoclonal antibody (sigma M2, isotype IgG1) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta actin mouse monoclonal antibody (60008-1-Ig, isotype IgM) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Mouse total IgG, Mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgA monoclonal antibodies, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) was used for detection. The result indicates that RGAM001 strongly binds to all Mouse IgGs, Mouse IgM and IgA as well as Rat IgG. It shows weak reactivity for pig IgG and does not react with other species tested in the experiment. 

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody (11749-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody (51067-2-AP) at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody (14528-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody (80001-1-RR) at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used for detection. The result indicates that RGAM001 is highly specific for rabbit IgG and does not react with other species tested in the experiment.

Various cell lysates were subject to SDS PAGE followed by western blot with anti-HSPA5 antibody (66574-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Vimentin antibody (60330-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Beta Catenin antibody (66379-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-GORASP2 antibody (66627-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).

WB of HeLa and HEK-293 Cell lysates (30 μg/lane): Various cell lysates were detected with anti-Beta Catenin 66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, 0.88 μL) (Lane 1 and Lane 2); Anti- GORASP2 (66627-1-Ig, Mouse IgG1, 0.18 μg) with FlexAble HRP Kit (KFA025, 0.36 μL) (Lane 3 and Lane 4); anti-Beta Catenin (66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, Linker 1.76 μL) with Quencher 0.88 μL, add 0.18 μg GORASP2 antibody as competitor antibody (Lane 5 and Lane 6). Primary antibody : Linker : Quencher=0.5 μg : 1 μL : 2 μL

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-594 (red).  Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-MARS antibody (67739-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Importin Alpha 5 antibody (67897-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-594 (red). Cell nuclei are in blue.

Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-AP, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-c-SRC antibody (60315-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 antibody (66777-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065) and Tyramide-594 (red). Cell nuclei are in blue. 

 Various cell lysates were subjected to SDS PAGE followed by western blot with anti-P53 antibody (10442-1-AP)  labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Lamin B1 antibody (12987-1-AP)  labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Vimentin antibody (10366-1-AP)  labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-GAPDH antibody (10494-1-AP)  labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide with anti-E-cadherin ​Antibody(20874-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005). Antibody used at a dilution of 1:1000 under 10x and 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide with anti-E-cadherin ​Antibody(20874-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005). Antibody used at a dilution of 1:1000 under 10x and 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GORASP2 antibody (10598-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-594 (red). Cell nuclei are in blue. 

Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-AP, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue. 

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.

Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.

HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60008 (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody HRP-60004 at a dilution of 1:10000.