1X10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD4 (65179-1-Ig, Clone:OX-35) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug Mouse IgG2a isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.