1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD1c (65176-1-Ig, Clone:L161) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19 (CL488-65197). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD1c (65176-1-Ig, Clone:L161) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
