1X10^6 PHA treated (3d) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 594 Anti-Human CD279 (CL594-65168, Clone:EH12.2H7) (right) or Mouse IgG1 Isotype Control (left). 1X10^6 untreated human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 594 Anti-Human CD279 (CL594-65168, Clone:EH12.2H7) (center). Cells were not fixed.
1X10^6 PHA treated (3d) human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD279 (CL594-65168, Clone:EH12.2H7) (red) or Mouse IgG1 Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD279 (CL594-65168, Clone:EH12.2H7) (black dashed). Cells were not fixed.