1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD14 and 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 Daudi cells were surface stained with 0.20 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were not fixed.
Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (65165-1-Ig, Clone: BU63 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD14 and 5 ul Cardinal Red™ Anti-Human CD86 (CR-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD86 (CR-65165, Clone:BU63) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite®405 Anti-Human CD86 (CL405-65165, Clone: BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite®405 Anti-Human CD86 (CL405-65165, Clone: BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human peripheral blood monocytes were surface stained with 5 ul CoraLite®488 Anti-Human CD86 (CL488-65165, Clone: BU63) (red), or Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were not fixed .
1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD14 and 5 ul CoraLite® Plus 555 Anti-Human CD86 (CL555-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD86 (CL555-65165, Clone:BU63) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with CL405 Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human CD86 (CL647-65165, Clone:BU63), or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD86 (CL647-65165, Clone:BU63), or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
