1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2), 1 ug Anti-Mouse CD134 (65136-1-Ig, Clone: OX-86) or rat IgG1 isotype control, and FITC anti-rat IgG1 Antibody at dilution 1:100. Cells were not fixed.