1x10^6 unstimulated or PMA and ionomycin stimulated (overnight) BALB/c mouse CD4+ T cells were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse CD154 (65129-1-Ig, Clone: MR1) and FITC anti-Armenian Hamster IgG at dilution 1:100. Cells were not fixed.
