1x10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface stained with 0.5 ug Anti-Mouse PD-1/CD279 (65120-1-Ig, Clone: J43.1) and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:100, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
