1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD44 (CL488-65117, Clone:IM7) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.