1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse TCR beta (65106-1-Ig, Clone:H57-597) and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (green), or stained with 0.5 ug isotype control antibody and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (black). Cells were not fixed.