1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.