1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.
