1X10^6 SJL mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45.1 (65092-1-Ig, Clone:A20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or not stained (blank) (black). Cells were not fixed .