1X10^6 Daudi cells were surface stained with 0.25 ug Anti-Human CD80 (B7-1) (65083-1-Ig, Clone: 2D10.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.