1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD45.2 (65072-1-Ig, Clone: 104) and FITC anti-Mouse IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.