1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and 0.5 ug FITC Plus Rat IgG2a isotype control (left) or 0.5 ug FITC Plus Anti-Mouse CD8a (FITC-65069, Clone: 53-6.7) (right). Cells were not fixed.