1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD54 (65066-1-Ig, Clone: YN1/1.7.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black). Cells were not fixed.