1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b / LAMP2 (65052-1-Ig, Clone: ABL-93) and FITC anti-Rat IgG2a Antibody (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).