Validation Data Gallery
WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis of zebrafish using 80008-1-RR (same clone as 80008-1-PBS)
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 80008-1-RR (same clone as 80008-1-PBS)
WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colorectal cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colorectal cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80008-1-RR (Alpha smooth muscle actin specific antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IF Staining of mouse small intestine using 80008-1-RR (same clone as 80008-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Alpha smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Alpha smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IF Staining of mouse embryo using 80008-1-RR (same clone as 80008-1-PBS)
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
FC experiment of C2C12 using 80008-1-RR (same clone as 80008-1-PBS)
1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
× 1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
80008-1-PBS targets Alpha smooth muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.
| Tested Reactivity | human, mouse, rat, pig, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2882938 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.