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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Sonic Hedgehog (SHH) protein

Activity

Typically ≤350 ng/mL

Species Reactivity

human,mouse

Purity

>90%

Publications(3)

Cat no : HZ-1306

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Validation Data Gallery

  • Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.

    Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.

  • Proteintech HEK293 derived SHH (HZ-1306) demonstrates higher activity as indicated by more 30-fold decrease in EC50. Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH. The EC50 was determined using a 4-parameter non-linear regression model.

    Proteintech HEK293 derived SHH (HZ-1306) demonstrates higher activity as indicated by more 30-fold decrease in EC50. Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH. The EC50 was determined using a 4-parameter non-linear regression model.

  • iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

    iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

Technical Specifications

GeneID 6469
Species Human
Expression HEK293
Activity Typically ≤350 ng/mL
Purity >90%
Endotoxin<1.0 EU/μg
Accession NumberQ15465
Molecular Mass 19-22 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched receptor (PTC), that in association with smoothened (SMO) activate the transcription of target genes. HumanKine SHH is a high-quality, animal-free recombinant Sonic Hedgehog protein designed for advanced stem cell and neurodevelopment research. In brain organoid differentiation, SHH plays a key morphogenic role by helping guide ventral patterning, supporting the generation of midbrain and ventral neural lineages, and enabling more physiologically relevant brain model development. HumanKine SHH expressed in HEK293 cells offers key advantages including native human conformation, high bioactivity, low endotoxin, xeno-free production, and strong lot-to-lot consistency. These features make it a reliable choice for researchers seeking reproducible results in organoid workflows, neural differentiation, and developmental signaling studies.

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Publications

SpeciesTitle
human

Mol Biol Rep

Discovery of YS-1 as a cell line of gastric inflammatory cancer-associated fibroblasts

Authors - Satoe Numakura
View Article

Int J Mol Sci

FAM20B-Catalyzed Glycosylation Regulates the Chondrogenic and Osteogenic Differentiation of the Embryonic Condyle by Controlling IHH Diffusion and Release

Authors - Xiaoyan Chen
View Article
rat

EBioMedicine

Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis

Authors - Shihui Xu
View Article
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