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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-18 protein- GMP grade

EC50

20-160 ng/mL

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1340-GMP

Synonyms

IGIF, IL1F4

Validation Data Gallery

  • GMP Recombinant human IL-18 (Cat no: HZ-1340-GMP) induces dose-dependent release of IFN-gamma in the KG-1 human cell line. KG-1 cells were treated with increasing concentration of recombinant IL-18 for 48 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 20 -160 ng/mL.

    GMP Recombinant human IL-18 (Cat no: HZ-1340-GMP) induces dose-dependent release of IFN-gamma in the KG-1 human cell line. KG-1 cells were treated with increasing concentration of recombinant IL-18 for 48 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 20 -160 ng/mL.

  • Purity of recombinant human IL-18 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-18 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Technical Specifications

GeneID 3606
Species Human
Expression HEK293
EC50 20-160 ng/mL
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberQ14116
Molecular Mass 19 kDa reduced, 19-21 kDa non-reduced, monomer, non-glycosylated
Formulation 1X PBS pH 7.4 + 0.5% CHAPS
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Human Interleukin-18 (IL-18) is a pro-inflammatory cytokine from the IL-1 family and a critical mediator of innate and adaptive immunity. IL-18 functions primarily by inducing IFN-γ production, supporting Th1 polarization, enhancing NK-cell cytotoxicity, and cooperating with IL-12 in pathogen defense and inflammatory signaling. IL-18 is broadly expressed as an inactive precursor in various tissues and cell types, especially those involved in immune surveillance and barrier defense. IL-18 is synthesized as pro-IL-18 and requires caspase-1 (inflammasome) processing for activation. This two-step regulation restricts its biological activity to inflammatory contexts while allowing broad basal expression.

Synonyms

IGIF, IL1F4

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