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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-10 Heat Stable protein

Activity

5-50 ng/mL

Species Reactivity

human

Purity

>95%

Cat no : HZ-1344

Synonyms

KGF2

Validation Data Gallery

  • Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 5-50 ng/mL.

    Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 5-50 ng/mL.

  • Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. HumanKine FGF-10 Heat Stable and product from Competitor A were reconstituted and pre-incubated in assay medium for 9 days at 37°C before bioassay. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model.

    Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. HumanKine FGF-10 Heat Stable and product from Competitor A were reconstituted and pre-incubated in assay medium for 9 days at 37°C before bioassay. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model.

  • Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. HumanKine FGF-10 Heat Stable was reconstituted and pre-incubated in assay medium for 9 days at 37°C before bioassay. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. Compared with freshly reconstituted FGF-10 Heat Stable protein, pre-incubated HumanKine FGF-10 retains the same potency.

    Recombinant human FGF-10 Heat Stable protein (Cat no: HZ-1344) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line in serum free medium. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. HumanKine FGF-10 Heat Stable was reconstituted and pre-incubated in assay medium for 9 days at 37°C before bioassay. MCF-7 cells were treated with increasing concentrations of recombinant FGF-10 Heat Stable for 96 hours. Compared with freshly reconstituted FGF-10 Heat Stable protein, pre-incubated HumanKine FGF-10 retains the same potency.

  • Purity of recombinant human FGF-10 Heat Stable was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-10 Heat Stable was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Technical Specifications

GeneID 2255
Species Human
Expression HEK293
Activity 5-50 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberO015520
Molecular Mass 16 and 50 kDa reduced, 28 and 50 kDa non-reduced, monomer and homodimer, glycosylated
Formulation 5X PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

Recombinant Human FGF-10 is a highly purified paracrine growth factor optimized for the expansion and structural maturation of complex 3D organoid cultures. By signaling through the FGFR2b receptor, this recombinant protein mimics the native mesenchymal niche to drive the robust proliferation, survival, and long-term self-renewal of epithelial progenitor cells. Essential for recapitulating authentic tissue architecture in vitro, Human FGF-10 actively stimulates localized epithelial budding and branching morphogenesis. It is an indispensable, bioactive media supplement for high-performance branching and glandular models, including distal lung, prostate, mammary, and gastrointestinal organoids.

Synonyms

KGF2

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