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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human R-Spondin-I protein

Activity

4-20 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1328

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Validation Data Gallery

  • Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human R-Spondin-1 (HZ-1328) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model.

    Recombinant human R-Spondin-1 (HZ-1328) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model.

Technical Specifications

GeneID 284654
Species Human
Expression HEK293
Activity 4-20 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession Number2QMKA7
Molecular Mass 36-50 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

R-Spondin 1 is an approximately 40kDA, glycosylated protein that serves as a major agonist of the Wnt signaling pathway. It regulates the turnover of the LRP6 co-receptor by preventing its internalization by DKK-1. R-Spondin 1 plays several roles in embryonic development including sex phenotype reversal, female sex determination, and ovarian differentiation. It also promotes stem cell turnover in the mammary glands, intestine, colon, and kidneys. Increased R-Spondin 1 expression has been linked to growth and migration of ovarian cancer cells as well as decreased sensitivity of gliomas to radiation-based therapies (PMID: 17804805, 22439850, 32749219, 30572097).

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Publications

SpeciesTitle
mouse

Nat Commun

Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells

Authors - Yang Sun
View Article
human

Mol Neurobiol

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis

Authors - Yipeng Zhao
View Article