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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-22 protein

Activity

0.045-0.225 ng/mL EC50

Species Reactivity

human

Purity

> 95%

Cat no : HZ-1325

Synonyms

IL-TIF, cytokine ZCYTO18

Validation Data Gallery

  • Purity of recombinant human IL-22 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-22 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-22 (Cat no: HZ-1325) induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

    Recombinant human IL-22 (Cat no: HZ-1325) induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

  • Proteintech IL-22 (Cat no: HZ-1325) demonstrates equivalent induction of IL-10 release and EC50 compared to a leading competitor. Recombinant human IL-22 induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

    Proteintech IL-22 (Cat no: HZ-1325) demonstrates equivalent induction of IL-10 release and EC50 compared to a leading competitor. Recombinant human IL-22 induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

Technical Specifications

GeneID 50616
Species Human
Expression HEK293
Activity 0.045-0.225 ng/mL EC50
Purity > 95%
Endotoxin<1.0 EU/μg
Accession NumberQ9GZX6
Molecular Mass 25-40 kDa reduced and non-reduced, dimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-22 is a 6-α-helix, bundle cytokine that is a member of the IL-10 family of cytokines. It is primarily produced by innate lymphoid cells (ILCS) and multiple T cell subsets including αβT, γδT, and NKT cells. Its production is regulated upstream by several different cytokines including IL-23, IL-1β, and IL-7. IL-22 binds with the IL-22 receptor, expressed on stromal and epithelial cells in a wide range of tissues, but it can also bind in circulation with the IL-22 binding protein (IL-22BP), which functions as a negative regulator. Binding with the receptor triggers several downstream pathways resulting in pro-inflammatory responses, increased mucus production, as well as increased production of anti-bacterial and anti-fungal proteins. IL-22 also plays a role in promoting wound healing and tissue regeneration in response to injury. Overexpression of IL-22 has been linked to several cancers, most notably colon cancer (PMID: 25706098, 33042126).

Synonyms

IL-TIF, cytokine ZCYTO18

Publications

SpeciesTitle
human

Skin Res Technol

Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis

Authors - Delu Che
View Article