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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-2 protein- GMP grade

EC50

0.05-0.35 ng/mL

Specific Activity

minimally 1.77 x 106 IU/mg, typically 8.63 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1015-GMP

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Validation Data Gallery

  • Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP-grade recombinant human IL-2 (HZ-1015-GMP) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.05-0.35 ng/mL.

    GMP-grade recombinant human IL-2 (HZ-1015-GMP) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.05-0.35 ng/mL.

Technical Specifications

GeneID 3558
Species Human
Expression HEK293
EC50 0.05-0.35 ng/mL
Specific Activityminimally 1.77 x 106 IU/mg, typically 8.63 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP60568
Molecular Mass 17 kDa reduced,16 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF