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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-19 protein

Activity

200-1000 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1332

Synonyms

NG.1, Melanoma differentiation-associated protein-like protein, Interleukin-19, MDA1, ZMDA1

Validation Data Gallery

  • Purity of recombinant human IL-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-19 (HZ-1332) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were treated with increasing concentrations of recombinant human PDGFbb under low serum conditions for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 200-1000 ng/mL.

    Recombinant human IL-19 (HZ-1332) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were treated with increasing concentrations of recombinant human PDGFbb under low serum conditions for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 200-1000 ng/mL.

Technical Specifications

GeneID 29949
Species Human
Expression HEK293
Activity 200-1000 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ9UHD0
Molecular Mass 25-32 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-19 (IL-19) is part of a smaller subfamily of interleukins that also includes IL-20, IL-22, and IL-24. These cytokines are all classified by their shared ability to bind and signal through the IL-20 receptor. IL-19 primarily exerts anti-inflammatory effects. It upregulates the production of IL-10 in PBMCs, particularly monocytes and dendritic cells and promotes the secretion of Th2-associated cytokine secretion from CD4+ T cells. It is thought to favor the polarization of naïve T cells into Th2s over Th1s, yielding a more anti-inflammatory phenotype. IL-19 also has several anti-inflammatory roles in vascular diseases, with its levels often increased in these instances to protect arteries from excessive damage (PMID: 29681905, 15827959).

Synonyms

NG.1, Melanoma differentiation-associated protein-like protein, Interleukin-19, MDA1, ZMDA1