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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 beta protein

Activity

Inquire

Species Reactivity

human

Purity

> 95%

Cat no : HZ-1321

Synonyms

Interleukin-12 subunit beta (IL-12B) Cytotoxic lymphocyte maturation factor (CLMF p40), IL-12 subunit p40, NK cell stimulatory factor chain 2 (NKSF2)

Validation Data Gallery

  • Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Technical Specifications

GeneID 3593
Species Human
Expression HEK293
Activity Inquire
Purity > 95%
Endotoxin< 0.1 EU/ug
Accession NumberP29460
Molecular Mass 37-50 kDa reduced, 37 to 47, 65 to 95, and 125 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS pH 7.4
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-12p40, also known as IL-12B, is a subunit of both the IL-12 and IL-23 cytokines. It is not thought to be bioactive on its own, but it has been shown to regulate IL-12 activity in antagonistic manner by competitively binding with the IL-12 receptor. IL-12p40 also functions as a chemoattractant molecule for macrophage infiltration into tumor sites. There has additionally been evidence to suggest that IL-12p40 mediates a protective immune response against mycobacterial infection by serving as a necessary component for granuloma formation and promoting Th1 effector cell functions (PMID: 17126601, 10453037, 11739515).

Synonyms

Interleukin-12 subunit beta (IL-12B) Cytotoxic lymphocyte maturation factor (CLMF p40), IL-12 subunit p40, NK cell stimulatory factor chain 2 (NKSF2)