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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-11 protein

Activity

10-50 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1333

Synonyms

AGIF, Interleukin 11, Adipogenesis inhibitory factor, Oprelvekin

Validation Data Gallery

  • Purity of recombinant human IL-11 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-11 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-11 (HZ-1333) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-11 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 10-50 ng/mL.

    Recombinant human IL-11 (HZ-1333) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-11 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 10-50 ng/mL.

Technical Specifications

GeneID 3589
Species Human
Expression HEK293
Activity 10-50 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberA8K3F7
Molecular Mass 20 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1 x PBS + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-11 (IL-11) is a member of the IL-6 family of cytokines. It is a pleiotropic cytokine that contributes to hematopoiesis, bone development, tissue remodeling, and tumor development. IL-11 binds with its receptor (IL-11RA), which is widely expressed on stromal cells including fibroblasts, smooth muscle cells, and adipocytes. It is thought to be a driver of pulmonary and dermal fibrosis. Upregulation of IL-11 has been linked to rheumatoid arthritis, ulcerative colitis, and Crohn’s disease.

Synonyms

AGIF, Interleukin 11, Adipogenesis inhibitory factor, Oprelvekin