• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-1 alpha protein

Activity

0.125-1.25 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1320

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM



Technical Specifications

GeneID 3552
Species Human
Expression HEK293
Activity 0.125-1.25 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP01583
Molecular Mass 22 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).

Synonyms

Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM

Publications

SpeciesTitle
human

Skin Res Technol

Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis

Authors - Delu Che